Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Treatment
Wild type and Knockout mouse tissue
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
2 µg/ml
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
KO samples from CPT2 knockout in muscle.
Exposure
10 minute(s) and 0 second(s)
Positive control
For the same protein samples, I used Anti-CPT2 Antibody from Sigma (Cat: ABS85) for western blot. This ABS85 antibody works very well for my samples. Strong specific band was detected in WT sample, but no band was detected in KO samples.
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in western blot and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 02 2018