Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Loading amount
100000 cells
Specification
Hela cells
Gel Running Conditions
Non-reduced Denaturing (NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/3000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk in PBS_0.1% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG (H+L) HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 73 kDa Non-specific: 35 kDa
Positive control
Whole cell extract of Hela cells. In addition to expected 73 kD band, a weak non-specific band around 35 kD can be also seen.
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投稿 May 14 2012