Anti-CPSF6 抗体 (ab99347)
Key features and details
- Rabbit polyclonal to CPSF6
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CPSF6 antibody
CPSF6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CPSF6 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
免疫原
Synthetic peptide, corresponding to a region between residue 1 and 50 of Human CPSF6 (NP_008938.1).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab99347 was affinity purified using an epitope specific to CPSF6 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99347の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 59 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 59 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3'-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping. -
配列類似性
Belongs to the RRM CPSF6/7 family.
Contains 1 RRM (RNA recognition motif) domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles. - Information by UniProt
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参照データベース
- Entrez Gene: 417844 Chicken
- Entrez Gene: 768231 Cow
- Entrez Gene: 11052 Human
- Entrez Gene: 432508 Mouse
- Entrez Gene: 100174771 Orangutan
- Entrez Gene: 299811 Rat
- Entrez Gene: 446580 Xenopus laevis
- Entrez Gene: 327069 Zebrafish
see all -
別名
- CFIM antibody
- CFIm68 antibody
- cleavage and polyadenylation specific factor 6, 68kDa antibody
see all
画像
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All lanes : Anti-CPSF6 antibody (ab99347) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 59 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF6 with ab99347 at 1/200 (1µg/ml). Detection: DAB.
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Detection of CPSF6 by Western Blot of Immunprecipitate. ab99347 at 1µg/ml staining CPSF6 in HeLa whole cell lysate immunoprecipitated using ab99347 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 1 seconds.
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IHC image of CPSF6 staining in human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99347, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab99347 は 8 報の論文で使用されています。
- Schwich OD et al. SRSF3 and SRSF7 modulate 3'UTR length through suppression or activation of proximal polyadenylation sites and regulation of CFIm levels. Genome Biol 22:82 (2021). PubMed: 33706811
- Chen S et al. The mRNA Export Receptor NXF1 Coordinates Transcriptional Dynamics, Alternative Polyadenylation, and mRNA Export. Mol Cell 74:118-131.e7 (2019). PubMed: 30819645
- Bochnakian A et al. Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA. J Virol 93:N/A (2019). PubMed: 31341054
- Bulli L et al. Complex Interplay between HIV-1 Capsid and MX2-Independent Alpha Interferon-Induced Antiviral Factors. J Virol 90:7469-80 (2016). PubMed: 27279606
- Dharan A et al. KIF5B and Nup358 Cooperatively Mediate the Nuclear Import of HIV-1 during Infection. PLoS Pathog 12:e1005700 (2016). PubMed: 27327622
- Rasheedi S et al. The Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Subunit of the Capsid-recruited Pre-messenger RNA Cleavage Factor I (CFIm) Complex Mediates HIV-1 Integration into Genes. J Biol Chem 291:11809-19 (2016). PubMed: 26994143
- Rasaiyaah J et al. HIV-1 evades innate immune recognition through specific cofactor recruitment. Nature 503:402-5 (2013). WB ; Human . PubMed: 24196705
- Hori T et al. A carboxy-terminally truncated human CPSF6 lacking residues encoded by exon 6 inhibits HIV-1 cDNA synthesis and promotes capsid disassembly. J Virol 87:7726-36 (2013). WB ; Human . PubMed: 23658440