Anti-CSN8 抗体 [EPR5139] (ab124779)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5139] to CSN8
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CSN8 antibody [EPR5139]
CSN8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5139] to CSN8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human CSN8 aa 150-250 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: Fetal liver, HepG2, SH SY5Y or HeLa cell lysates IHC-P: Human brain tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5139 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124779の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ターゲット情報
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機能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
配列類似性
Belongs to the CSN8 family.
Contains 1 PCI domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10920 Human
- Entrez Gene: 108679 Mouse
- Entrez Gene: 363283 Rat
- Omim: 616011 Human
- SwissProt: Q99627 Human
- SwissProt: Q8VBV7 Mouse
- SwissProt: Q6P4Z9 Rat
- Unigene: 531713 Human
see all -
別名
- COP9 homolog antibody
- COP9 signalosome complex subunit 8 antibody
- COP9 signalosome subunit 8 antibody
see all
画像
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ab124779, at 1/100, staining CSN8 in formalin fixed paraffin embedded Human brain tissue using immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CSN8 antibody [EPR5139] (ab124779) at 1/1000 dilution
Lane 1 : Fetal liver cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : SH SY5Y cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124779 は 1 報の論文で使用されています。
- Zhang H et al. Identification of novel dipeptidyl peptidase 9 substrates by two-dimensional differential in-gel electrophoresis. FEBS J 282:3737-57 (2015). PubMed: 26175140