Anti-COL1A2 抗体 (ab96723)
Key features and details
- Rabbit polyclonal to COL1A2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-COL1A2 antibody
COL1A2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to COL1A2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Dog, Monkey -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1057 and 1339 of COL1A2 (NP_000080).
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ポジティブ・コントロール
- WB: 293T and A431 cell lysate ICC/IF: A431 cell
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96723の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/500 - 1/3000. Predicted molecular weight: 129 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 129 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Type I collagen is a member of group I collagen (fibrillar forming collagen). -
組織特異性
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. -
関連疾患
Ehlers-Danlos syndrome 7B
Osteogenesis imperfecta 1
Osteogenesis imperfecta 2
Ehlers-Danlos syndrome, autosomal recessive, cardiac valvular form
Osteogenesis imperfecta 3
Osteogenesis imperfecta 4
A chromosomal aberration involving COL1A2 may be a cause of lipoblastomas, which are benign tumors resulting from transformation of adipocytes, usually diagnosed in children. Translocation t(7;8)(p22;q13) with PLAG1. -
配列類似性
Belongs to the fibrillar collagen family.
Contains 1 fibrillar collagen NC1 domain. -
ドメイン
The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function. -
翻訳後修飾
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 282188 Cow
- Entrez Gene: 403824 Dog
- Entrez Gene: 1278 Human
- Entrez Gene: 12843 Mouse
- Omim: 120160 Human
- SwissProt: P02465 Cow
- SwissProt: O46392 Dog
- SwissProt: P08123 Human
see all -
別名
- Alpha 2 collagen type I antibody
- Alpha 2 type I collagen antibody
- Alpha 2 type I procollagen antibody
see all
画像
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All lanes : Anti-COL1A2 antibody (ab96723) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 129 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
Lower image: merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab96723 は 46 報の論文で使用されています。
- Liu C et al. The mechanosensitive lncRNA Neat1 promotes osteoblast function through paraspeckle-dependent Smurf1 mRNA retention. Bone Res 10:18 (2022). PubMed: 35210394
- Yang BY et al. Porous Se@SiO2 Nanoparticles Enhance Wound Healing by ROS-PI3K/Akt Pathway in Dermal Fibroblasts and Reduce Scar Formation. Front Bioeng Biotechnol 10:852482 (2022). PubMed: 35387298
- Pane K et al. Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study. Int J Mol Sci 23:N/A (2022). PubMed: 35409352
- Meng X et al. MicroRNA-34a and microRNA-146a target CELF3 and suppress the osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch. J Dent Sci 17:1281-1291 (2022). PubMed: 35784124
- Ju N et al. Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice. Sci Rep 12:12474 (2022). PubMed: 35864207