Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary mouse neural stem cells)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
5 µg
Treatment
differentiated in B27 media for 48 h
Specification
Primary mouse neural stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBS
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL femto
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
Positive control
Lane 2, 48 hour differentiated neural stem cell lysates.
Additional data
Additional Notes
Lane1, lysates from mouse neural stem cells in proliferation condition. Lane2, 48 hour differentiated neural stem cell lysates.
Lanes 3 - 5, 72 - 120 hour differentiated neural stem cell lysates.
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投稿 Jun 18 2015