Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse cell lines)
Loading amount
20 µg
Specification
Mouse cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, PBS-Tween 0.1%
Secondary antibody
Secondary antibody
None used
Detection
Detection method
Supersignal west dura
Exposure
8 minute(s) and 0 second(s)
Bands
Specific: 20 kDa Non-specific: 65 kDa
Additional data
Additional Notes
Order: (1) MEF, (2) F9, (3) GC-1, (4) NIH-3T3
Detects a species of the predicted mobility in a range of common mouse cell lines.
Detects a species of the predicted mobility in a range of common mouse cell lines.
Abcam response
We would like to thank the reviewer for submitting this Abreview for product. We are glad that this reviewer was able to achieve such good results in mouse. Further reduction of background might be achieved by using a backgrounf reducing buffer ab64234 or by reducing the concentration of the antibody,
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 23 2012