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All lanes : Anti-CLOCK antibody (ab43106) at 1/1000 dilution
Lane 1 : a total protein extract from E coli with 50ng to 100ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM (H+L), HRP conjugated, at 1/5000 dilution
Predicted band size : 95 kDa
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