Anti-CLK2 抗体 (ab86147)
Key features and details
- Rabbit polyclonal to CLK2
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CLK2 antibody
CLK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CLK2 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa whole cell lysate. ICC: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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ICC |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
ICC
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates serines, threonines and tyrosines. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylates on all three types of residues. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1196 Human
- Entrez Gene: 12748 Mouse
- Entrez Gene: 365842 Rat
- Omim: 602989 Human
- SwissProt: P49760 Human
- SwissProt: O35491 Mouse
- Unigene: 73986 Human
- Unigene: 288098 Mouse
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別名
- CDC like kinase 2 antibody
- CDC-like kinase 2 antibody
- CLK 2 antibody
see all
画像
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ab86147 staining CLK2 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab86147 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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ab86147 staining CLK2 in human colon carcinoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/30 in PBS + 1% BSA) for 16 hour at 4°C. A FITC-conjugated goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.
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Anti-CLK2 antibody (ab86147) at 0.5 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Additional bands at: 36 kDa, 64 kDa, 94 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Human Dual specificity protein kinase CLK2 contains a number of potential phosphorylation sites (SwissProt) which may explain the banding pattern at a higher molecular weight than predicted (64 kDa).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab86147 は 3 報の論文で使用されています。
- Zhou Q et al. Targeting CLK3 inhibits the progression of cholangiocarcinoma by reprogramming nucleotide metabolism. J Exp Med 217:N/A (2020). PubMed: 32453420
- Iwai K et al. Anti-tumor efficacy of a novel CLK inhibitor via targeting RNA splicing and MYC-dependent vulnerability. EMBO Mol Med 10:N/A (2018). PubMed: 29769258
- Petsalaki E & Zachos G Clks 1, 2 and 4 prevent chromatin breakage by regulating the Aurora B-dependent abscission checkpoint. Nat Commun 7:11451 (2016). PubMed: 27126587