Anti-CKS1 抗体 (ab72639)
Key features and details
- Rabbit polyclonal to CKS1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-CKS1 antibody -
製品の詳細
Rabbit polyclonal to CKS1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide derived from the N terminus of human CKS1.
-
ポジティブ・コントロール
- Extracts from Jurkat cells, treated with serum (20%, 15mins). HeLa cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72639の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa).
|
|
ICC/IF |
1/500 - 1/1000.
|
特記事項 |
---|
WB
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 10 kDa). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
-
機能
Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. -
配列類似性
Belongs to the CKS family. - Information by UniProt
-
参照データベース
- Entrez Gene: 1163 Human
- Entrez Gene: 54124 Mouse
- Omim: 116900 Human
- SwissProt: P61024 Human
- SwissProt: P61025 Mouse
- Unigene: 374378 Human
- Unigene: 3049 Mouse
-
別名
- CDC2 associated protein CKS1 antibody
- CDC28 protein kinase 1 antibody
- CDC28 protein kinase 1B antibody
see all
画像
-
All lanes : Anti-CKS1 antibody (ab72639) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells, treated
with serum (20%, 15mins)
Lane 2 : Extracts from Jurkat cells, treated
with serum (20%, 15mins) with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 10 kDa
Observed band size: <19 kDa why is the actual band size different from the predicted? -
ab72639, at 1/500 dilution, staining CKS1 in HeLa cells by Immunofluorescence in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab72639 は 3 報の論文で使用されています。
- Li P et al. Knockdown of LINC00657 inhibits the viability, migration and invasion of pancreatic cancer cells by regulating the miR-520h/CKS1B axis. Exp Ther Med 22:1142 (2021). PubMed: 34504588
- Li Y et al. Histone Deacetylase Inhibitor Trichostatin A Reduces Endothelial Cell Proliferation by Suppressing STAT5A-Related Gene Transcription. Front Oncol 11:746266 (2021). PubMed: 34650929
- Zeng Z et al. Downregulation of CKS1B restrains the proliferation, migration, invasion and angiogenesis of retinoblastoma cells through the MEK/ERK signaling pathway. Int J Mol Med 44:103-114 (2019). PubMed: 31115482