Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS cells)
Loading amount
20 µg
Specification
U2OS cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.1% Tween + 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Sheep anti mouse-HRP
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 29 kDa Non-specific: none kDa
Negative control
CIP29 siRNA knockdown
Additional data
Additional Notes
Secondary antibody must be incubated with presence of 5% milk. Otherwise background bands appear.
1= whole cell extract
2= whole cell extract of CIP29 siRNA transfected cells
1= whole cell extract
2= whole cell extract of CIP29 siRNA transfected cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Nicole Verkaik
Verified customer
投稿 Feb 09 2010