Application
Immunocytochemistry
Sample
Fruit fly (Drosophila melanogaster) Cell (DM2 cells)
Specification
DM2 cells
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1
Other product details
Dilution
1/50
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: FITC-labelled affinity purified donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Fixation in 4% PFA for 5 minutes.
Incubation with primary antibody (ab10887) was 18 hours at 4 degrees C or 2 hours at room temperature.
Incubation with primary antibody (ab10887) was 18 hours at 4 degrees C or 2 hours at room temperature.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Beth Sullivan
Verified customer
投稿 Oct 05 2005