Anti-CHMP4A 抗体 (ab67058)
Key features and details
- Mouse polyclonal to CHMP4A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CHMP4A antibody -
製品の詳細
Mouse polyclonal to CHMP4A -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human CHMP4A aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- transfected 293T cells lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67058の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview)
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 25 kDa. Use BSA for blocking (see Abreview) |
ターゲット情報
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関連性
Component of the ESCRT-III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-III complex is probably involved in the concentration of MVB cargo. In the ESCRT-III complex, it probably serves as an acceptor for ESCRT-I complex on endosomal membranes. In case of infection, the HIV-1 virus takes advantage of the ESCRT-III complex for budding and exocytic cargos of viral proteins, via the association of CHMP4 proteins with PDCD6IP/AIP1, a protein directly recruited by HIV-1 p6 protein that functions at sites of viral Gag assembly and budding. -
細胞内局在
Cytoplasmic: Cytoplasmic vesicle membrane. Membrane-associated - localizes to large vesicle-like structures. -
参照データベース
- Entrez Gene: 29082 Human
- GenBank: BC010893 Human
- Omim: BC010893 Human
- SwissProt: Q9BY43 Human
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別名
- C14orf123 antibody
- Charged multivesicular body protein 4a antibody
- Chromatin modifying protein 4a antibody
see all
画像
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All lanes : Anti-CHMP4A antibody (ab67058) at 1/500 dilution
Lane 1 : CHMP4A transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 25 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab67058 は 2 報の論文で使用されています。
- Jia J et al. Galectin-3 Coordinates a Cellular System for Lysosomal Repair and Removal. Dev Cell 52:69-87.e8 (2020). PubMed: 31813797
- Arii J et al. ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity. Nat Commun 9:3379 (2018). PubMed: 30139939