Application
Western blot
Sample
Mouse Cell lysate - nuclear (mouse and human cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse and human cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 300 kDa
Positive control
Jurkat cells as human cells
G1E2 cells as murine cells
Additional data
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species. The antibody did detect the human cell line sample as expected.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 20 2016