Anti-CHD4 抗体 [3F2/4] (ab70469)
Key features and details
- Mouse monoclonal [3F2/4] to CHD4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CHD4 antibody [3F2/4]
CHD4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3F2/4] to CHD4 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details
適用なし: IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide corresponding to Human CHD4.
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ポジティブ・コントロール
- IHC: Human breast carcinoma tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
3F2/4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70469の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 5 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. -
配列類似性
Belongs to the SNF2/RAD54 helicase family.
Contains 2 chromo domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 PHD-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > centrosome. Associates with centrosomes in interphase. - Information by UniProt
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参照データベース
- Entrez Gene: 1108 Human
- Entrez Gene: 107932 Mouse
- Omim: 603277 Human
- SwissProt: Q14839 Human
- SwissProt: Q6PDQ2 Mouse
- Unigene: 162233 Human
- Unigene: 333388 Mouse
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別名
- ATP dependent helicase CHD4 antibody
- ATP-dependent helicase CHD4 antibody
- CHD 4 antibody
see all
画像
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IHC image of CHD4 staining in human breast carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70469, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab70469 は 46 報の論文で使用されています。
- Chrysanthou S et al. The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity. Nucleic Acids Res 50:3169-3189 (2022). PubMed: 35150568
- Garipler G et al. The BTB transcription factors ZBTB11 and ZFP131 maintain pluripotency by repressing pro-differentiation genes. Cell Rep 38:110524 (2022). PubMed: 35294876
- Dufour CR et al. The mTOR chromatin-bound interactome in prostate cancer. Cell Rep 38:110534 (2022). PubMed: 35320709
- Pantier R et al. SALL4 controls cell fate in response to DNA base composition. Mol Cell 81:845-858.e8 (2021). PubMed: 33406384
- Lüdtke TH et al. Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung. Respir Res 22:85 (2021). PubMed: 33731112