Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (7.5)
Sample
Human Cell lysate - whole cell (Hela cells)
Specification
Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Sigma A2304
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: 100 kDa
Exposure
2 minute(s) and 26 second(s)
Negative control
Depletion of CHD1L by 3 different siRNA. The Knockdown has been performed for 3 days at a concentration of 16 nM.
Positive control
HeLa total cell lysate
Additional data
Additional Notes
If interested I can supply you with the siRNA sequence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Peter Rüthemann
Verified customer
投稿 Jul 04 2013