Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Nervus Suralis peripheral neurons)
Permeabilization
Yes - Triton X-100
Specification
Nervus Suralis peripheral neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Fixative
Acetone
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Diluent: PBS+4%FCS+4%NGS+0,4% Triton X-100
Dilution
1/50
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Cy™3 AffiniPure Donkey Anti-Mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Sample:
Teased fibers of a biopsy of human Nervus Suralis.
Fixation: 10 Minutes in -20°C Acetone
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Thomas Klein
Verified customer
投稿 Sep 11 2015