Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Fibroblasts)
Loading amount
40 µg
Specification
Fibroblasts
Treatment
virus
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk TTBS
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti-mouse HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 02 2008