Anti-CEP128 抗体 (ab118797)
Key features and details
- Rabbit polyclonal to CEP128
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CEP128 antibody
CEP128 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CEP128 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to a region within amino acids 1044-1094 of Human CEP128 (NP_689659.2).
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ポジティブ・コントロール
- 293T and HeLa whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118797の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 128 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Associates with the mother centriole. - Information by UniProt
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参照データベース
- Entrez Gene: 145508 Human
- SwissProt: Q6ZU80 Human
- Unigene: 162889 Human
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別名
- C14orf145 antibody
- C14orf61 antibody
- CE128_HUMAN antibody
see all
画像
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All lanes : Anti-CEP128 antibody (ab118797) at 1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 128 kDa
Exposure time: 3 minutes -
Detection of CEP128 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118797 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab118797 は 2 報の論文で使用されています。
- Chong WM et al. Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages. Elife 9:N/A (2020). PubMed: 32242819
- Viol L et al. Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. J Cell Biol 219:N/A (2020). PubMed: 32211891