Anti-CENPI 抗体 (ab118796)
Key features and details
- Rabbit polyclonal to CENPI
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CENPI antibody
CENPI 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CENPI -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human CENPI aa 50-150.
Database link: Q92674 -
ポジティブ・コントロール
- HeLa, 293T and Jurkat whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118796の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 87 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 87 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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関連性
CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore. -
細胞内局在
Cytoplasmic and Nuclear -
参照データベース
- Entrez Gene: 2491 Human
- Omim: 300065 Human
- SwissProt: Q92674 Human
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別名
- CENP I antibody
- centromere protein I antibody
- Follicle simulating hormone primary response protein antibody
see all
画像
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All lanes : Anti-CENPI antibody (ab118796) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 87 kDa
Exposure time: 30 seconds -
Detection of CENPI in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118796 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab118796 は 4 報の論文で使用されています。
- Wang J et al. Centromere Protein I (CENP-I) Is Upregulated in Gastric Cancer, Predicts Poor Prognosis, and Promotes Tumor Cell Proliferation and Migration. Technol Cancer Res Treat 20:15330338211045510 (2021). PubMed: 34617858
- Engqvist H et al. Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry. Front Oncol 10:162 (2020). PubMed: 32133296
- Kyriacou E & Heun P High-resolution mapping of centromeric protein association using APEX-chromatin fibers. Epigenetics Chromatin 11:68 (2018). PubMed: 30445992
- Thangavelu PU et al. Overexpression of the E2F target geneCENPIpromotes chromosome instability and predicts poor prognosis in estrogen receptor-positive breast cancer. Oncotarget 8:62167-62182 (2017). PubMed: 28977935