Anti-CENPH 抗体 (ab88593)
Key features and details
- Mouse polyclonal to CENPH
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CENPH antibody
CENPH 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to CENPH -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein within Human CENPH aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- HeLa cell lysate. Raw 264.7 cell lysate. NIH/3T3 cell lysate. Lysate from transfected 293T cells. HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88593の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. -
配列類似性
Belongs to the centromere protein H family. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase. - Information by UniProt
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参照データベース
- Entrez Gene: 64946 Human
- Entrez Gene: 26886 Mouse
- Omim: 605607 Human
- SwissProt: Q9H3R5 Human
- SwissProt: Q9QYM8 Mouse
- Unigene: 631967 Human
- Unigene: 273502 Mouse
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別名
- CENP H antibody
- CENP-H antibody
- CENPH antibody
see all
画像
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Anti-CENPH antibody (ab88593) at 5 µg/ml + HeLa cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Anti-CENPH antibody (ab88593) at 5 µg/ml + Raw 264.7 cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
Anti-CENPH antibody (ab88593) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa
Additional bands at: ~19 kDa, ~60 kDa, ~70 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-CENPH antibody (ab88593) at 5 µg/ml
Lane 1 : cell lysate from CENPH transfected 293T cells
Lane 2 : cell lysate from non transfected 293T cells
Lysates/proteins at 25 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 28 kDa -
ab88593 at 10 µg/ml detecting CENPH in HeLa cells by immunofluorescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab88593 は 2 報の論文で使用されています。
- Hu L et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment. Nucleic Acids Res 47:468-479 (2019). PubMed: 30407575
- Zhang T et al. CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes. Development 144:305-312 (2017). PubMed: 27993978