Application
Flow Cytometry
Sample
Human Cell (T98G glioma cells)
Specification
T98G glioma cells
Fixation
Ethanol
Permeabilization
Yes - Ethanol
Gating Strategy
intact cells with >80% and <250% of G1 DNA content
Other product details
Dilution
1/400
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS/2% BSA
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 488 goat anti-rabit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jan 29 2008