Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Lymphoblast chromosomes)
Specification
Lymphoblast chromosomes
Fixative
Carnoy (Methanol:acetic acid 3:1)
Permeabilization
Yes - 0,25% Triton X-100 in blocking buffer
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 1x + 3% milk + 0,25% Triton X-100
Secondary antibody
Name
Non-Abcam antibody was used: Rhod (TRITC) AffiniPure Donkey Anti Rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: TRITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/200
Additional data
Additional Notes
Cells were counterstained with DAPI
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Marie-Chantal Grégoire
Verified customer
投稿 Sep 30 2008