Application
Immunocytochemistry
Sample
Human Cultured Cells (HT1080 Cell line)
Specification
HT1080 Cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 546 Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/1000
Additional data
Additional Notes
Staining is exclusively nuclear
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Neil Taylor
Verified customer
投稿 Oct 06 2009