Anti-CEACAM1 抗体 [EPR4049] (ab108397)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4049] to CEACAM1
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CEACAM1 antibody [EPR4049]
CEACAM1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4049] to CEACAM1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal liver, A549, SW480, and HepG2 cell lysates; IHC-P: Human kidney clear cell carcinoma tissue. IP: SW480 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4049 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108397の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 58 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 58 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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配列類似性
Belongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted; Cell membrane and Cell membrane. Localizes to sites of cell-cell contact. - Information by UniProt
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参照データベース
- Entrez Gene: 634 Human
- Omim: 109770 Human
- SwissProt: P13688 Human
- Unigene: 512682 Human
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製品の状態
There are 11 isoforms produced by alternative splicing. Isoform 1 = BGPa; CEACAM1-4L; TM1-CEA; Isoform 2 = BGPg; CEACAM1-4C1; Isoform 3 = BGPh; CEACAM1-3; Isoform 4 = BGPi; CEACAM1-3C2; Isoform 5 = BGPy; CEACAM1-3AL; Isoform 6 = BGPb; CEACAM1-3L; TM2-CEA; Isoform 7 = BGPx; CEACAM1-1L; Isoform 8 = BGPc; CEACAM1-4S; TM3-CEA; Isoform 9 = BGPz; CEACAM1-3AS and Isoform 11 = BGPd; CEACAM1-3S. -
別名
- Antigen CD66 antibody
- BGP 1 antibody
- BGP antibody
see all
画像
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All lanes : Anti-CEACAM1 antibody [EPR4049] (ab108397) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : CEACAM1 knockout A549 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108397 observed at 100-180 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108397 Anti-CEACAM1 antibody [EPR4049] was shown to specifically react with CEACAM1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267046 (knockout cell lysate ab257388) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab108397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human clear cell carcinoma of kidney tissue sections labeling CEACAM1 with Purified ab108397 at 1:80 dilution (3.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CEACAM1 antibody [EPR4049] (ab108397) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : CEACAM1 knockout A549 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab108397 observed at 100-180 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108397 Anti-CEACAM1 antibody [EPR4049] was shown to specifically react with CEACAM1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267047 (knockout cell lysate ab257389) was used. Wild-type and CEACAM1 knockout samples were subjected to SDS-PAGE. ab108397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-CEACAM1 antibody [EPR4049] (ab108397) at 1/10000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 100-180 kDa why is the actual band size different from the predicted?The smear and multiple bands are consistent with what have been described in the literature PMID: 17071610, 26448580 and 30050594, due to a variety of splice variants and/or differential glycosylation
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ab108397 (purified) at 1:20 dilution (1.5µg) immunoprecipitating CEACAM1 in SW480 whole cell lysate.
Lane 1 (input): SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108397 & SW480 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108397 in SW480 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
Unpurified ab108397 at 1/50 dilution staining CEACAM1 in Human kidney tissue by Immunohistochemistry, Paraffin-embedded tissue.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab108397 は 7 報の論文で使用されています。
- Liu D et al. Transfer RNA-derived fragment 5'tRF-Gly promotes the development of hepatocellular carcinoma by direct targeting of carcinoembryonic antigen-related cell adhesion molecule 1. Cancer Sci 113:3476-3488 (2022). PubMed: 35879647
- Mattox AK et al. Myeloid Cells Are Enriched in Tonsillar Crypts, Providing Insight into the Viral Tropism of Human Papillomavirus. Am J Pathol 191:1774-1786 (2021). PubMed: 34303699
- Chean J et al. Human CEACAM1-LF regulates lipid storage in HepG2 cells via fatty acid transporter CD36. J Biol Chem 297:101311 (2021). PubMed: 34666041
- Suzuki S et al. Immune-checkpoint molecules on regulatory T-cells as a potential therapeutic target in head and neck squamous cell cancers. Cancer Sci 111:1943-1957 (2020). PubMed: 32304268
- Xu D et al. PD-L1 Expression Is Regulated By NF-?B During EMT Signaling In Gastric Carcinoma. Onco Targets Ther 12:10099-10105 (2019). PubMed: 31819504
- Wei Y et al. DcR3 promotes proliferation and invasion of pancreatic cancer via a DcR3/STAT1/IRF1 feedback loop. Am J Cancer Res 9:2618-2633 (2019). PubMed: 31911850
- Ye S et al. Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1. Sci Rep 8:15468 (2018). PubMed: 30341336