Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic stem cells)
Permeabilization
Yes - Triton 0.5%
Specification
embryonic stem cells
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking - PBS+BSA+milk
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Abcam response
Thank you for your honest feedback. To our knowledge, this antibody has not been previously tested in ICC. Based on data presented in this review, ab70289 may not be useful for immunocytochemistry. It may be, however, that protocol changes eg. Changes in blocking, permeabilization and primary conc. /dilution could be helpful to get specific staining of the target.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Shai Melcer
Verified customer
投稿 Nov 18 2010