Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (retina)
Specification
retina
Permeabilization
Yes - triton X
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: bsa blocking Solution
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: invitrogen Alexafluor conjugated goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/100
Additional data
Additional Notes
No specific staining in the retina at 4dpf.
Abcam response
Thank you for your valuable feedback. ab64533 has not been previously tested in Zebrafish and your data suggests that ab64533 may not detect CDC42 in Zebrafish under these experimental conditions. We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ryan Macdonald
Verified customer
投稿 May 22 2014