Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Specification
Pancreas
Blocking step
FBS / BSA in TBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% FBS / 1% BSA in TBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Envision System (Leyca)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Abcam response
Thank you for your feedback. We are sorry to hear that this antibody has not given you good results in immunohistochemistry in paraffin embedded sections.
Unfortunately we had not previously tested this application in our lab, and it may well be this antibody is not suitable for this application. We would welcome more feedback from our customer in order to make any further decision about the application suitability.
For users wishing to test our products in untested species or applications, we would encourage them to participate in our Abtrial program, by which you will beneficiate from a discount off your next purchase for the value of the product tested by submitting your results to our website.
The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Raul Peña
Verified customer
投稿 Dec 03 2012