Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow cells smear)
Specification
Bone marrow cells smear
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin 0.5%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Other product details
Dilution
1/300
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dako antibody diluent + serum 0.1%
Secondary antibody
Name
Non-Abcam antibody was used: Molecular Probe Goat anti-rat
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Dilution
1/400
Additional data
Additional Notes
Bone marrow was directly smeared on glass slides.
Dapi stains nuclei in blue.
Image was taken on an epifluorescence microscope (x60)
Dapi stains nuclei in blue.
Image was taken on an epifluorescence microscope (x60)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Sophie Nadaud
Verified customer
投稿 Feb 23 2012