Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Amnion Epithelial Cells)
Permeabilization
Yes - Triton-X
Specification
Amnion Epithelial Cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: overnight while rocking
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Alexa-Fluor
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 594
Additional data
Additional Notes
The secondary antibody was at 1:400 in PBS, incubated room temp rocking for 30 min.
Immunofluorescence imaging for colocalization of pro-senescence marker P-p38MAPK (green) and CD9 (red). DAPI is the nuclear stain.
PubMed ID
27333275: View
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投稿 Sep 15 2017