Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (Gradient Gel)
Sample
Hamster Cell lysate - whole cell (CHO cells)
Specification
CHO cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% non-fat milk dry milk in 1x TBS-T
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase conjugated Goat Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL Luminata crescendo
Bands
Specific: 28 kDa
Exposure
45 minute(s) and 0 second(s)
Positive control
Hela Cells
Additional data
Additional Notes
Lane 1: CHO cell lysate
Lane 2: GC-1 cell lysate
Lane 3: HeLa cell lysate
Lane 4: COS-7 cell lysate
Lane 5: SH-SY5Y cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Soraia Martins
Verified customer
投稿 Mar 16 2015