Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Heart)
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Fixative
Acetone
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Goat Anti Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
This antibody did not give specific staining when used with PFA fixed cryosections. But works better when fixed with actone for 10min in -20¯ temperature. Washes after primary antibody incubation was done 10min*3 with 0,5%BSA with 1xPBS.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Dec 18 2017