Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
mouse uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Donkey Anti mouse
Host species: Donkey
Clonality: Polyclonal
Conjugation: Rhodamine
Host species: Donkey
Clonality: Polyclonal
Conjugation: Rhodamine
Additional data
Additional Notes
No specific staining. Negative control shows similar staining pattern as positive control.
Left: Positive control. Right: Negative Control
Abcam response
Thank you so much for testing this antibody in mouse. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species.
The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 16 2017