製品の概要

  • 製品名
    Anti-CD62P antibody [AK-6]
    CD62P 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AK-6] to CD62P
  • 特異性
    MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.
  • アプリケーション
    適用あり: Flow Cyt, IHC-P, IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Human, Rhesus monkey

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P 1/500 - 1/2000.
IHC-Fr 1/1000 - 1/5000.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • 組織特異性
    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • 関連疾患
    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • 配列類似性
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Antigen CD62 antibody
    • CD62 antibody
    • CD62 antigen-like family member P antibody
    • CD62P antibody
    • GMP 140 antibody
    • GMP-140 antibody
    • GMP140 antibody
    • GMRP antibody
    • Granule membrane protein 140 antibody
    • Granulocyte membrane protein antibody
    • GRMP antibody
    • LECAM 3 antibody
    • LECAM3 antibody
    • Leukocyte endothelial cell adhesion molecule 3 antibody
    • Leukocyte-endothelial cell adhesion molecule 3 antibody
    • LYAM3_HUMAN antibody
    • P Selectin antibody
    • P-selectin antibody
    • PADGEM antibody
    • Platelet activation dependent granule-external membrane protein antibody
    • Platelet alpha granule membrane protein antibody
    • PSEL antibody
    • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
    • SELP antibody
    see all

画像

  • ab6632 at 1/10 dilution staining human Renal Carcinoma tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Folowing heat mediated antigen retrieval the tissue was stained with antibody for 12 hours and then detected using a biotinylated donkey anti-mouse IgG antibody.

    This image is courtesy of an Abreview submitted on 3 March 2006.

    See Abreview

  • ab6632 staining CD62P in thronbin activated human platelets by Flow Cytometry analysis.

参考文献

This product has been referenced in:
  • Könnecke M  et al. Immune imbalance in nasal polyps of caucasian chronic rhinosinusitis patients is associated with a downregulation of e-selectin. J Immunol Res 2014:959854 (2014). IHC-Fr ; Human . Read more (PubMed: 24995349) »
  • Assassi S  et al. Skin gene expression correlates of severity of interstitial lung disease in systemic sclerosis. Arthritis Rheum 65:2917-27 (2013). IHC-P ; Human . Read more (PubMed: 23897225) »

See all 4 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (nasal mucosa)
Specification
nasal mucosa
Fixative
Acetone
Permeabilization
No
Blocking step
H2O2 as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C
Username

Mr. Michael Könnecke

Verified customer

投稿 Jan 07 2013

Thank you for contacting us.

ab6632 has yet not been tested in ELISA application; this however doesn't mean this product is not suitable, the product might be suitable however we do not have data to confirm it.

On the other hand produ...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I have issued a free of charge replacement.

Please note that this free of charge repla...

Read More

Thank you for your enquiry regarding ab6632. Unfortunately this antibody has not been epitope mapped so I cannot tell you exactly which amino acids it recognises. However this clone, AK-6, was studied as part of the Adhesion Structures section of L...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Specification
Kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Mar 03 2006

Thank you for your enquiry. This antibody is specific for CD62P. To our knowledge it has not been tested for cross-reactivity with E-selectin (CD62E). As discussed in the reference given below, this antibody was raised against a human platelet glyc...

Read More

This antibody has been shown to cross-react with human and Rhesus monkey. Other species have not been tested. This antibody is suitable for use on formalin fixed tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録