Anti-CD45RA 抗体 [MEM-56] (ab8217)
Key features and details
- Mouse monoclonal [MEM-56] to CD45RA
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
製品の概要
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製品名
Anti-CD45RA antibody [MEM-56]
CD45RA 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-56] to CD45RA -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD45RA. Thymocytes and T lymphocytes
Database link: P08575-8 -
ポジティブ・コントロール
- WB: HUT-78 human cutaneous T cell lymphoma cell lysate.
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特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
MEM-56 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use a concentration of 0.3 - 4 µg/ml.
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特記事項 |
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WB
Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use a concentration of 0.3 - 4 µg/ml. |
ターゲット情報
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機能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
関連疾患
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
ドメイン
The first PTPase domain interacts with SKAP1. -
翻訳後修飾
Heavily N- and O-glycosylated. -
細胞内局在
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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別名
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
画像
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Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RA with ab8217 at 0.3 ug/ml showing separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
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Immunohistochemical analysis of Paraffin-embedded sections Human Tonsil tissue labelling CD45RA with ab8217 at 5µg/ml.
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Lane 1 : CD45 control antibody
Lanes 2-3 : Anti-CD45RA antibody [MEM-56] (ab8217)
Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
Lane 2 : sample of cell lysate prepared under non-reducing conditions
Lane 3 : sample of cell lysate prepared under reducing conditions
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab8217 は 4 報の論文で使用されています。
- Zizzari IG et al. The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions. PLoS One 10:e0132617 (2015). PubMed: 26147970
- Abdulamir AS et al. Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma. BMC Immunol 9:73 (2008). ICC ; Human . PubMed: 19087256
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480
- Bazil V et al. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression. Immunogenetics 29:202-5 (1989). PubMed: 2466778