Application
Flow Cytometry
Sample
Human Cell (HUCPVCs)
Specification
HUCPVCs
Preparation
Cell harvesting/tissue preparation method: Cell dissociation buffer
Sample buffer: Enzyme free
Sample buffer: Enzyme free
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
Live cells
Other product details
Dilution
1/100
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS + 0.2% BSA + 0.1% Sodium azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rat IgG PE
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/100
Additional data
Additional Notes
This antibody is not listed as an antibody that cross reacts with human CD44 but I tried it out of curiosity. The poor rating, therefore, represents its efficacy on human cells only.
The purple plot represents rat IgG isotype control and the green plot indicates CD44 [KM81].
The purple plot represents rat IgG isotype control and the green plot indicates CD44 [KM81].
Abcam response
Thank you so much for testing this antibody with human samples. As far as we know, this has not been tried before. We appreciate very much your efforts, but are sorry to hear that the antibody did not give satisfactory results with this species. We would welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 13 2011