Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (RAW 264.7)
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Specification
RAW 264.7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS
Dilution
1/200
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: Alexa Flour488 Chicken Anti Rabbit IgG (H+L)
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
WGA - Alexa Flour568 Wheat Germ Agglutinin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mahesh Shivananjappa
Verified customer
投稿 May 23 2012