Application
Western blot
Sample
Human Cell lysate - other (Human B chronic lymphocytic leukaemia cells)
Loading amount
18 µg
Specification
Human B chronic lymphocytic leukaemia cells
Gel Running Conditions
Reduced Denaturing (12% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 30 second(s)
Bands
Specific: 42 kDa
Additional data
Additional Notes
Sub-cellular fractionation of B-CLL cells was performed (method: Maloney, Am J Physiol, 1998). Lane 1 - nuclear fraction, lane 2 - cytoskeletal fraction, lane 3 - membrane fraction, lane 4 - cytosolic fraction.
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投稿 Feb 22 2008