Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate, pH 6
Permeabilization
No
Specification
Kidney
Blocking step
Background Punisher Reagent, Biocare Medicals as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS - 50mM
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Biocare Medicals - HRP MACH 4 Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Morgan Thompson
Verified customer
投稿 Nov 28 2016