Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (brain)
Specification
brain
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: KPBS 0.02M 3% NDS 0.1% TritonX
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: alexa 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 680
Additional data
Additional Notes
Cy5 channel gives a lot a background noise. Staining should be better with an alexa 488 (GFP) or 594 (Texas red).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 28 2017