Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung tissue)
Specification
lung tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - MeOH
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Other product details
Dilution
1/250
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 594 goat Anti-Rat
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/500
Additional data
Additional Notes
I had problems with non-specific binding of the secondary antibody, so it was difficult to determine specific binding.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 10 2007