Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with 0.05% Trypsin-EDTA
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 4°C · Diluent: FACS buffer (0.5% BSA and 2mM EDTA)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: R-Phycoerythrin Horse Anti-Mouse
Host species: Horse
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Horse
Clonality: Polyclonal
Conjugation: Phycoerythrin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Andrea Cabrera
Verified customer
投稿 Feb 06 2017