Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen retrieval solution pH 9 of DAKO
Permeabilization
No
Specification
Lung
Blocking step
Fetal calf serum (filtered) as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: LSAB 2 Kit of DAKO
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Additional data
Additional Notes
For results it needed a high concentration of primary antibody and an uncommonly long incubation time with AEC of 20min.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Thibault Geinoz
Verified customer
投稿 Jun 27 2015