Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (MDA-MB-231 xenograft model)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid
Permeabilization
No
Specification
MDA-MB-231 xenograft model
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: SuperPicture™ Polymer Detection Kit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Immunostaining of anti-CD31 antibody (ab76533 blue) in a paraffin embedded MDA-MB-231 xenograft model of a nude mouse using ab76533. Note negative staining of vascular endothelial cells in the center of the image.
Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. We are sorry to hear that this antibody has not given you good results using mouse sample. This antibody has not been tested with this species before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 01 2017