Application
Immunocytochemistry
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/100
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed
Additional data
Additional Notes
Cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Under these conditions, the antibody does not produce the expected membrane-associated staining pattern. MeOH fixed samples were also evaluated, but did not produce the expected staining pattern.
Abcam response
Thank you for your valuable feedback. ICC/IF was removed as a guaranteed application in 2016. Following this ab76533 has been retested in ICC/IF and we have confirmed that the new lots are suitable for this application. We lot test this antibody in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Jan 16 2017