Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Muscle)
Permeabilization
Yes - Triton and Tween
Specification
Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBS (Overnight)
Dilution
1/100
Additional data
Additional Notes
I have tried to upload two pictures, one with positive staining from the CD31 antibody with Alexa Fluor 594 (see below) and the other which was stained as a control with the absence of the primary antibody to investigate the specificity.
There was no visible staining present upon the no primary antibody control. Exposures were increased for up to 2 seconds to ensure that either no detection or positive detection was effectively identified. High exposures were required to identify positive detection and the brightness of the stain was low.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 27 2016