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Western blot - CD31 antibody [EPR3094] (ab76533)
![Western blot - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/images/cd31a.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/images/cd31b.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/images/cd31c.jpg)
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Product Name
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CD31 antibody [EPR3094]
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CD31 抗体 (46件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3094] to CD31
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Immunogen
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Synthetic peptide corresponding to residues in the extracellular domain of human CD31
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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Flow Cyt, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions Flow Cyt: 1/50. IHC-P: 1/250 - 1/500. Antigen retrievalis recommended before commencing with IHC staining protocol. IP: 1/20. WB: 1/5000 - 1/20000. Predicted molecular weight: 83 kDa.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: THP-1 and Jurkat cell lysates IHC-P: Human kidney tissue and human muscle tissue
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Cellular localization
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Membrane; single pass type I membrane protein. Cell junction
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Research areas
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Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Thrombocytic Lineage Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules Cancer >> Tumor immunology >> CD markers Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules Cardiovascular >> Angiogenesis >> Angiogenic Factors Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial Cardiovascular >> Blood >> Platelets
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Relevance
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CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
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CD31 antibody [EPR3094] (ab76533)の別名
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3094
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - その理由
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Produced under U.S. Patent No. 5,675,063.
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Abcam では全ての製品情報を一元管理しており、 CD31 antibody [EPR3094] について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for CD31 antibody [EPR3094] images, references, products related to ab76533 and other tools.
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CD31 antibody [EPR3094] images:
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Western blot - CD31 antibody [EPR3094] (ab76533)
All lanes : CD31 antibody [EPR3094] (ab76533) at 1/20000 dilution
Lane 1 : THP-1 cell lysate Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 83 kDa Observed band size : 125 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
Immunohistochemical analysis of paraffin embedded human kidney tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
Immunohistochemical analysis of paraffin embedded human muscle tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
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Search PubMed (MEDLINE) for references to CD31
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CD31 antibody [EPR3094] - more information
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Customer reviews (feedback) regarding CD31 antibody [EPR3094] |
Customer FAQs regarding CD31 antibody [EPR3094] |
Protocols for CD31 antibody [EPR3094] |
Price and availability of products related to CD31 antibody [EPR3094] |
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CD31 antibody [EPR3094] - related products:
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CD31 antibody [EPR3094] - other tools:
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Contact Abcam with a Technical Enquiry about CD31 antibody [EPR3094] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"