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Read our guarantee »Products:Cardiovascular >> Blood >> Platelets
Anti-CD31 antibody [EPR3094]
CD31 抗体 (50件) 一覧
Rabbit monoclonal [EPR3094] to CD31
WB, IP, Flow Cyt, IHC-Pmore details
Reacts with
Human
Does not react with
Mouse, Rat
Synthetic peptide corresponding to residues in the extracellular domain of human CD31
WB: THP-1 and Jurkat cell lysates IHC-P: Human kidney tissue and human muscle tissue
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Tissue culture supernatant
Monoclonal
EPR3094
IgG
Cardiovascular >> Cardiovascular Markers >> Cell Markers >> Endothelial Cells
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Thrombocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Tumor immunology >> CD markers
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Cardiovascular >> Blood >> Platelets
Our Abpromise guarantee covers the use of ab76533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/20000.Predicted molecular weight: 83 kDa.
IP: 1/20
Flow Cyt: 1/50
IHC-P: 1/250 - 1/500.Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.(We suggest to program the pressure cooker to run for 30 seconds at 125° C, followed by 10 seconds at 90° C. Then let the slide cool down to room temperature (10 - 20 minutes). )
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
Target information above from: UniProt accessionP16284
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - CD31 antibody [EPR3094] (ab76533)
![Western blot - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/Images/76/ab76533/cd31a.jpg)
All lanes : Anti-CD31 antibody [EPR3094] (ab76533) at 1/20000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 83 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/Images/76/ab76533/cd31b.jpg)
Immunohistochemical analysis of paraffin embedded human kidney tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/Images/76/ab76533/cd31c.jpg)
Immunohistochemical analysis of paraffin embedded human muscle tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/images/76/ab76533/CD31-Primary-antibodies-ab76533-3.jpg)
ab76533 staining CD31 in human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% serum for 30 minutes at 20°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at20°C. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.
Image courtesy of an anonymous Abreview.
This product has been referenced in:
See 1 publication for this product
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![Western blot - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/Images/76/ab76533/cd31a.jpg)
All lanes : Anti-CD31 antibody [EPR3094] (ab76533) at 1/20000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 83 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/Images/76/ab76533/cd31b.jpg)
Immunohistochemical analysis of paraffin embedded human kidney tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/Images/76/ab76533/cd31c.jpg)
Immunohistochemical analysis of paraffin embedded human muscle tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - CD31 antibody [EPR3094] (ab76533)](/ps/datasheet/images/76/ab76533/CD31-Primary-antibodies-ab76533-3.jpg)
ab76533 staining CD31 in human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% serum for 30 minutes at 20°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at20°C. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.
Image courtesy of an anonymous Abreview.
![CD31 antibody [EPR3094] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (76533)](/ps/datasheet/images/76/ab76533/CD31-Primary-antibodies-ab76533-2.jpg)
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