CD31 antibody [EPR3094] (ab76533)

Product Name 

CD31 antibody [EPR3094]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EPR3094] to CD31

Immunogen 

Synthetic peptide corresponding to residues in the extracellular domain of human CD31

Reacts with 

Hu

Does not react with 
(species key)

Ms, Rat

Tested applications 
(see key)

Flow Cyt, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/50.
IHC-P: 1/250 - 1/500. Antigen retrievalis recommended before commencing with IHC staining protocol.
IP: 1/20.
WB: 1/5000 - 1/20000. Predicted molecular weight: 83 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: THP-1 and Jurkat cell lysates
IHC-P: Human kidney tissue and human muscle tissue

Cellular localization 

Membrane; single pass type I membrane protein.
Cell junction

Research areas 

Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Thrombocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Neutrophil Lineage
Stem Cells >> Hematopoietic Progenitors >> Myeloid >> Monocytic Lineage
Stem Cells >> Hematopoietic Progenitors >> Lymphoid >> T Lymphocytic Lineage
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Cell adhesion molecules
Cancer >> Tumor immunology >> CD markers
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Cardiovascular >> Angiogenesis >> Angiogenic Factors
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Cell Adhesion Molecules >> Endothelial
Cardiovascular >> Blood >> Platelets

Relevance 

CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.

CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EPR3094

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - その理由

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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