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ich hoffe jetzt ist die Sequenz mit dabei, wenn nicht bitte nochmal |
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ANSWER: |
Vielen Dank für die zwei Sequenzen von Lachs (Salmo salar). |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-CD31 antibody [EPR3094] (ab76533) at 1/20000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 83 kDa
Observed band size : 125 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin embedded human kidney tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
Immunohistochemical analysis of paraffin embedded human muscle tissue using ab76533 at a 1/250 dilution. Note positive staining of endothelial cells.
ab76533 staining CD31 in human muscle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then blocked with 3% serum for 30 minutes at 20ºC followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at20ºC. A Cy3®-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.
Image courtesy of an anonymous Abreview.
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