Application
Western blot
Sample
Cow Tissue lysate - whole (bovine epididymis)
Loading amount
40 µg
Specification
bovine epididymis
Treatment
homogenized in 0.02 M Hepes buffer, pH 7.0, containing 1% octylglucoside and a complete protease inhibitor cocktail tablet
Gel Running Conditions
Reduced Denaturing (7,5% gel)
Blocking step
0.05 M Tris buffer containing 0.15 M NaCl, 0.1% Tween 20, 5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.05M Tris,0.15M NaCl,0.1% Tween20,5%BSA
Secondary antibody
Name
Non-Abcam antibody was used: HRP- conjugated goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 116 kDa
Positive control
lane 3: bovine epididymis homogenate
lane 4: human DPPIV
lane 4: human DPPIV
Negative control
lane 1: molecular weight standards
lane 2: bovine testis homogenate
lane 5: recombinant DPP8
lane 6: bovine DPP9
lane 7: molecular weight standards
lane 2: bovine testis homogenate
lane 5: recombinant DPP8
lane 6: bovine DPP9
lane 7: molecular weight standards
Additional data
PubMed ID
19188489: View
Additional Notes
nitrocellulose membrane
the primary antibody was incubated for 1 hr at room temperature and overnight at 4°C
the primary antibody was incubated for 1 hr at room temperature and overnight at 4°C
Abcam response
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Veronique Dubois
Verified customer
投稿 Aug 05 2009