Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS plus 1xcasein
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Texas goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Texas Red ®
Additional data
Abcam response
Thank you for your valuable feedback. We are pleased to see this has worked well in canine samples and IHC-Fr, both of which are new for this antibody.
We welcome any further feedback from other customers who have used ab113289 in canine samples and IHC-Fr which will help to establish how well this antibody works in this application and species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 01 2016